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Improving Biodiesel Production Through A Dynamic Sensor-Regulator System (DSRS) This system, manufactured by Zhang et al., incorporates both naturally occurring and synthetic bio pathways in Escherichia coli. The process seeks to convert glucose, a molecule naturally metabolized in E. coli, through a series of steps into fatty acid ethyl ester (FAEE), a usable biofuel. FAEE is “an excellent diesel fuel replacement due to its low water solubility and high energy density, and is suitable for microbial production because of its low toxicity to host cells.”〔http://ql3dq7xx6q.search.serialssolutions.com.offcampus.lib.washington.edu/?genre=article&issn=09581669&title=Current+Opinion+in+Biotechnology&volume=22&issue=6&date=20111201&atitle=Metabolic+engineering+of+microbial+pathways+for+advanced+biofuels+production.&spage=775&pages=775-783&sid=EBSCO:Academic+Search+Complete&aulast=Zhang〕 This process is revolutionary in the field of synthetic biology as it is the first synthetic sensor-regulator system to dynamically regulate the production of a commercial product, a biofuel, based on concentrations of intermediate molecules in the process. This regulation allows for an optimal output of FAEE by controlling output rates of each of the steps in the process which works to reduce bottlenecks, buildup of toxic chemicals, and production of unnecessary chemicals. In the future, Zhang et al. seek to apply this system to the production of other commercial compounds and to bring this FAEE producing system to large scale production. ==Module Schematic== ''FadR/Acyl-CoA Gene Regulation Schematic:'' Figure 1: Gene regulation by FadR, the product of the naturally occurring biosensor gene fadR, was tested prior to insertion in the E. coli genome. The promoter sequence that allowed for optimal efficiency was determined by testing a series of 20 strains of E. coli. Each strain contained a different promoter sequence, all with varying FadR binding strength, taken from a promoter library. These promoters were placed upstream of a gene coding for red fluorescent protein (rfp). Each strain was subsequently exposed to the same concentration of fatty acids. Optimal promoter strength and dynamic range, found in strains W and Y were then selected based on how fluorescent each strain was. The same test was conducted for FAEE production using the same promoters, with a similar result: strains W and Y produced the most FAEE. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Improving biodiesel production through a dynamic sensor regulator system」の詳細全文を読む スポンサード リンク
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